Quantification of cytokines

IL-6 was measured using ELISA mouse Ready-set-Go kit (Thermo Fisher Scientific; 88-7064-22, RRID:AB_2574986) as per the manufacturer’s instructions and read on a Safire II plate reader (Tecan). 2-Plex ProcartaPlex (Thermo Fisher Scientific; EPX02A-22187-901) was used to measure the concentrations of IFN-α/β, and the ProcartaPlex Cytokine and Chemokine Mouse 36-Plex (Thermo Fisher Scientific; EPXR360-26092-901) was used to assess the concentrations of 36 cytokines (IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-12p70, IL-13, IL-18, TNFα, IL-9, IL-10, IL-17A [CTLA-8], IL-22, IL-23, IL-27, G-CSF [CSF-3], IFN-α, IL-3, IL-15/IL-15R, IL-28, IL-31, IL-1α, LIF, ENA-78 [CXCL5], M-CSF, Eotaxin [CCL11], GROα [CXCL1], IP-10 [CXCL10], MCP-1 [CCL2], MCP-3 [CCL7], Mip-1α [CCL3], Mip-1β [CCL4], Mip-2, and RANTES [CCL5]), read on a Luminex 100 (Bio-Rad; RRID:SCR_018025).