The levels of interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin 6 (IL-6), interleukin 17a (IL-17a), and interleukin 10 (IL-10) in mouse serum were measured by enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems Minnesota, USA) according to the manufacturer's instructions. The concentrations of these cytokines were calculated from the standard curves.
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