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Analysis of Signaling Pathway Activation
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Platelets were pre-incubated with various concentrations of the non-reversible inhibitor of NF-κB, Bay 11-7082 (2 μM) (Sigma-Aldrich) or Ro 106-9920 (4 μM) (Tocris Bioscience), for 60 min at 37°C (23). Platelets were then stimulated with Pam3CSK4 or TRAP in the presence or absence of an anti-human TLR2 blocking MoAb to detect NF-κB signal modulation. Platelet supernatants were collected to determine soluble factors levels as outlined above.
Copyright and License information: ©2017 Damien, Cognasse, Payrastre, Spinelli, Blumberg, Arthaud, Eyraud, Phipps, McNicol, Pozzetto, Garraud and Hamzeh-Cognasse.
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