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Residues 1 to 314 of AMPKα1 were amplified from the construct pWZL-Neo-Myr-Flag-PRKAA1 (Addgene, Cambridge, MA) (33) and then cloned to pCMR2 lentiviral vector to express the constitutively activated AMPKα1 (AMPK-CA). Recombinant lentiviruses of the empty vector (pWZL-Neo-Myr-Flag-DEST) or AMPK-CA were obtained as described above.
Copyright and License information: ©2016, American Society for Microbiology. All Rights Reserved.
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