Data are expressed as the mean ± SEM and are representative of 2–4 independent experiments. The results from individual experiments were not combined because they were analyzed individually. The means from the different groups were compared by ANOVA followed by Tukey's honest significant difference (HSD) test. Comparisons between two groups were determined using Student's t-test. Analyses were performed using Prism 5.0 software (GraphPad). Statistical significance was set at P < 0.05.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.