Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

RNA sequencing.

JF Jeffrey A. Freiberg
YB Yoann Le Breton
BT Bao Q. Tran
AS Alison J. Scott
JH Janette M. Harro
RE Robert K. Ernst
YG Young Ah Goo
EM Emmanuel F. Mongodin
DG David R. Goodlett
KM Kevin S. McIver
MS Mark E. Shirtliff
request Request a Protocol
ask Ask a question
Favorite

The resulting cDNA libraries were submitted to the University of Maryland Institute for Bioscience and Biotechnology Research (UM-IBBR) Sequencing Facility located at the University of Maryland—College Park. Sequencing data in the Sanger FastQ format were generated using an Illumina HiSeq 1500 system in rapid-run mode (100-nucleotide [nt], single-end reads). Biological triplicates were sequenced for each time point.

Copyright and License information:  ©2016 Freiberg et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A