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Genetic diversity analysis, demographic history analysis and neutrality test

CZ Canglin Zhang
RY Rui Yang
LW Linbo Wu
CL Chunhai Luo
XG Xiaofang Guo
YD Yan Deng
HZ Hongning Zhou
YZ Yilong Zhang
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Intra- and interspecific ITS2/COII divergences were tested using the Kimura’s 2-parameter (K2P) distance model [39] in MEGA X [33]. Genetic divergence was determined using Nei’s distance model [40], in Arlequin v3.5.2.2 [41]. Genetic diversity indices were calculated and neutrality tests (Fu’s Fs [42] and Tajima’s D [43]) were performed using DnaSP v5.10 [44]. The mismatch distribution (with the simulation in Arlequin v.3.5) was achieved to distinguish a multimodal or ragged distribution from a smooth unimodal distribution [45–47].

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