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Total RNA was isolated, cDNA was synthesized from cells, and real-time RT-PCR was performed as described previously (6, 32). The levels of expression of mRNA were normalized to the housekeeping gene β-actin. The sequences of primers and probe sets for detection of β-actin, CXCL8, CXCL10, and CCL26 are listed in Table E1 in the online supplement. Primer and probe sets for PTPN2, -6, -10, and -11 and PAR-2 were purchased from Life Technologies (Grand Island, NY).
Copyright and License information: ©2016 by the American Thoracic Society
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