Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Autophagosome visualization by transmission electron microscopy
Request a Protocol
Ask a
question
Favorite
Tumor cells were obtained by trypsinization in paraffin-embedded tissues, washed with PBS and fixed in ice-cold 3% glutaraldehyde buffered at pH 7.4 with 0.1 M cacodylate for 24 h. The cells were then fixed in 1.0% OsO4, dehydrated in a progressive ethanol and acetone solution, and embedded in Epon 812 in sequence. Sections (1-μm thick) were cut and stained with uranium tetraacetate followed by lead citrate trihydrate. Subsequently, the sections were observed with a H-600 IV transmission electron microscopy and photographed.
Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
