Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Negative stain electron microscopy
Request a Protocol
Ask a
question
Favorite
Negative-staining EM allowed purity and concentration assessment, as well as adapting the T5 tail:FhuA ratio prior to cryo-grid preparation. Negative-stain grids of all samples were prepared using the mica-carbon flotation technique over a 2% sodium silicotungstate solution and were observed with an FEI/Thermo Fisher Scientific Tecnai 12 microscope (LaB6 electron source, 120 kV) equipped with a Gatan Orius 1000 CCD camera.
Copyright and License information: The Author(s) ©2017
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
