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Crystallisation

CA Charles-Adrien Arnaud
GE Grégory Effantin
CV Corinne Vivès
SE Sylvain Engilberge
MB Maria Bacia
PB Pascale Boulanger
EG Eric Girard
GS Guy Schoehn
CB Cécile Breyton
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Initial hits were obtained on the high throughput crystallisation platform on site. Crystals were reproducibly obtained at 4 °C by the hanging drop vapour diffusion setup by mixing 1:1 (v/v) protein solution (8 mg mL−1) with reservoir buffer containing 10% 3350 PEG, 0.2 M HEPES pH 7.5, 0.3 M LiSO4, 0.1 mM MnCl2. Selenomethionine-pb6 crystals were obtained with a protein solution at 7 mg mL−1 and a reservoir buffer containing 11% 3350 PEG, 0.2 M HEPES pH 7.5, 0.1 M LiSO4, 40 mM MnCl2. Crystals typically grew within 2 days. All crystals were cryoprotected with 25% PEG 400 and flash-frozen in liquid nitrogen using nylon loops (CryoLoopTM, Hampton Research).

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