2.10. Statistical analysis

Statistical analysis was calculated by SPSS 22.0 software (IBM Corp) and data are expressed as the mean ± SD. Student's t‐test and variance test were chosen for comparisons among groups. The Dunn's post hoc test was used to compare ANGPTL4 expression in the stroma of different human gallbladder lesion tissue specimens. The correlation between GBC patient clinicopathological characteristics and ANGPTL4 expression was assessed by χ² test or Fisher's exact test. Survival comparisons were performed by log‐rank test. The significance of various parameters for survival was determined by the Cox regression model. Each experiment was repeated at least three times. p < 0.05 was considered to be statistically significant.