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HepG2 cells were maintained in DMEM high-glucose medium (glucose; 25 mM) supplemented with FBS (10% v/v), L-glutamine (4 mM), sodium pyruvate (1 mM), and HEPES (1 mM). All cells were incubated at 37 °C and 5% CO2. Cells were used up to passage 20.
Copyright and License information: ©2016 The Author 2016. Published by Oxford University Press on behalf of the Society of Toxicology.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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