Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Examination of synergistic/antagonistic effects of drug combinations

SD Sergey A. Dyshlovoy
RM Ramin Madanchi
JH Jessica Hauschild
KO Katharina Otte
WA Winfried H. Alsdorf
US Udo Schumacher
VK Vladimir I. Kalinin
AS Alexandra S. Silchenko
SA Sergey A. Avilov
FH Friedemann Honecker
VS Valentin A. Stonik
CB Carsten Bokemeyer
GA Gunhild von Amsberg
request Request a Protocol
ask Ask a question
Favorite

Determination of synergistic, antagonistic, or additive effects of compounds used in combination assays was performed using the Chou-Talalay method as previously described [23]. The combinational index (CI) was calculated with the CompuSyn v.1.0. Software (ComboSyn, Inc., Paramus, NJ, USA). Fa (fraction affected) is defined as the non-survival fraction at a certain dose of compounds or their combinations. Synergism is defined as a CI < 0.85, whereas antagonism has a CI > 1.2. A CI of 0.85 to 1.2 is considered an additive effect.

Copyright and License information: The Author(s). ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A