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Microplastic and Macroplastic size detection and totals

EL Esperanza Huerta Lwanga
JV Jorge Mendoza Vega
VQ Victor Ku Quej
JC Jesus de los Angeles Chi
LC Lucero Sanchez del Cid
CC Cesar Chi
GS Griselda Escalona Segura
HG Henny Gertsen
TS Tamás Salánki
MP Martine van der Ploeg
AK Albert A. Koelmans
VG Violette Geissen
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MPs from soil, earthworm casts and chicken feces were counted with the use of a stereo microscope (Leica, objective10 × 21, Fig. S3) and particle size distribution was determined with a microscope (Leica, objective 40x/0.67, Fig. S4).

The floating MP (<5 mm) and MaP (5–150 mm) particles from the crop and gizzard of the chicken were placed in a glass petri dish for measurement which was carried out with the help of a millimetric paper adapted to the stereoscope. The characterization of the plastic debris was done visually.

All the plastic extractions were done in sterile conditions, using glass petri dishes, following the normal procedures of standard laboratories (using white coats and hats).

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