2.3. Cell viability and survival assay

For cell viability assay, cells (1 × 10⁴ cells/mL/100 μL/well) were seeded in a 96‐well plate. After overnight incubation, cells were treated with dasatinib (0, 0.1, 1, and 10 µM) for 24 hours. Cells were then washed twice with PBS, and viability was determined using MTS reagent according to the manufacturer's protocol. Briefly, eight µl of the culture media (RMPI‐1640 or DMEM) and 20 µL of MTS solution was added to each well, and the plate was incubated at 37℃ for 1 hour. The absorbance of each well was measured at 490 nm using a microplate reader (SPECTRA max 340PC; Molecular Devices, LLC). For cell survival, cells (2 × 10⁵ cells/ml/500 μL/well) were seeded in a 24‐well plate overnight. Cells were treated with dasatinib (0, 0.1, 1, and 10 µM) for 24 hours. Cells were then washed twice with PBS. The number of survived cells, which cannot be stained with trypan blue dye, was counted using a phase‐contrast microscope. The cell count assay was performed in triplicate. Data are mean ± standard error (SE) of three independent experiments. Survival is expressed as a percentage of control.