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Hippocampal organotypic slice (350 μm) cultures were prepared from postnatal day 4–7 rats through a McIllwain tissue chopper as described by Kim and colleagues51. Plasmids were biolistically transfected into slices at DIV 4–5 by using Gene-Gun (Bio-Rad, CA, USA). GluA1 plasmids were transfected together with a plasmid enconding enhanced green fluorescent protein (EGFP) to identify the transfected neurons. LTP experiments were performed 2–4 days later.
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