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Sampling of tear fluid and lacrimal fluid for proteomics

JJ Jae Hun Jung
YJ Yong Woo Ji
HH Ho Sik Hwang
JO Jae Won Oh
HK Hyun Chang Kim
HL Hyung Keun Lee
KK Kwang Pyo Kim
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To measure and collect the fluids from patients’ tears, a bonded 2.0 × 10-mm polyester fiber rod (TRANSORB® WICKS, FILTRONA, Richmond, VA, USA) was used as previously reported. Briefly, to collect the TF which is a mixed fluid of the secretion from the LGs, meibomian glands, and corneoconjunctival cells, a polyester wick was applied to the tear meniscus of the lower lid margin. After then, it was removed and placed into a 1.5-ml Eppendorf tube, which was stored at −70 °C until the mass spectrophotometric assay was performed. In addition to the TF, pure LF was collected. The detailed methods for collecting TF and LF were published with video file44.

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