Materials

The prothrombin neutralizing anti-human monoclonal antibody {"type":"entrez-nucleotide","attrs":{"text":"AB730006","term_id":"429901813","term_text":"AB730006"}}AB730006 ba SP14-046 IgG2b was obtained from MedImmune (Cambridge, UK).

Blood samples intended for in vitro studies at AstraZeneca R&D, Gothenburg, Sweden were drawn from 5 healthy volunteers (3 males and 2 females) after informed consent and approval from the local Gothenburg ethical committee (ethical permit number: 033-10). The sample size was not determined from a formal power calculation for this particular study but was based on previous experience of the assay methods used. Whole blood was collected into polypropylene tubes from Sarstedt (Nümbrecht, Germany) by free flow from a 17-gauge Venflon needle from Becton Dickinson Infusion Therapy AB (Helsingborg, Sweden). The first 2 mL of the collected blood was discarded. Then nine volumes blood was mixed with one volume 0.11 M trisodium citrate. Of the 50 mL whole blood collected per donor 10 mL was used for preparation of platelet poor plasma by centrifugation of the citrated blood at 2000 x g in a swing-out rotor for 20 min at 20 °C prior to transfer of the plasma supernatant to a new sample tube.