Solubility of LAPHs was carried out over a wide range of pH values from pH 2.0 to pH 10.0 as described by Tsumura et al. (2005). After appropriate dilution, the nitrogen contents in the supernatants were determined by the Biuret method (Gornall et al. 1949). Solubility analysis was carried out in triplicate. The nitrogen solubility of LAPHs, defined as the amount of soluble nitrogen from the total nitrogen, was calculated as follows:
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