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HepG2 cells were cultured in DMEM medium containing 10% fetal bovine serum (FBS) supplemented with penicillin and streptomycin and maintained at 37 °C in a 5% CO2 atmosphere, and were incubated for 24 h. Then, cells were washed twice with PBS, and the medium was changed to serum-free DMEM containing the antibiotics. After one-hour incubation, the cells were treated with drug, respectively, according to each experiment.
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