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We used the Gateway system (Invitrogen) to prepare N-terminal and C-terminal fusion constructs using pSPE3::Venus (a gift from P. Lemaire), pSPE3::Rfp1, pSPE3::Cherry, pSPE3::tomato for all constructs except PH::GFP which was cloned into pRN3. For construct details please refer to our previous methods publication38. All Kif2 constructs were prepared using Phallusia mammillata Kif2 (unique gene ID: phmamm.g00002556) and Ciona intestinalis Kif2 (unique gene ID: Ciinte.g00008837). All synthetic mRNAs were transcribed and capped with mMessage mMachine kit (Ambion).
Copyright and License information: The Author(s) ©2017
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