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Cells (3 × 105cells ) were seeded into 6 well cell culture plates and cultured in DMEM containing 10% FBS to near confluence. The confluent monolayer was carefully wounded and cellular debris was washed gently with PBS. The wounded monolayer was incubated in DMEM containing 10% FBS, 20 µg/ml fibronectin and DMSO or 75, 100, 150, 200 or 250 µg/ml extract for 24 hours. Migrating cells were examined under 10 × by phase contrast microscope (Olympus, Tokyo, Japan).
Copyright and License information: ©2016 The Korean Nutrition Society and the Korean Society of Community Nutrition
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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