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Flow cytometry was used to estimate total bacterial abundance. Samples were preserved by adding borax-buffered and filter sterilized formaldehyde to 2% final concentration. For flow cytometry, 200 μl of each preserved sample was stained with 20 μl of 5 mM Syto 13 (Invitrogen®) for 10 min (del Giorgio et al., 1996). Cells were counted using a flow cytometer (Cyflow Space, Partec). Two replicates were analyzed for each sample.
Copyright and License information: ©2017 Samad and Bertilsson.
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