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Two-dimensional PAGE analysis
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Two-dimensional PAGE analysis to assay Tsa1 sulfinylation was performed using pre-cast 18 cm nonlinear pH 3–10 IPG strips (Immobiline DryStrip, number 17-1235-01, GE Healthcare, Uppsala, Sweden) in the first dimension, 13.5% Duracryl (Millipore) polyacrylamide gels as the second dimension and silver staining to detect total protein22.
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