Identification of CENP-A, CENP-C, NUF2 and DAD1 genes in C. tropicalis

The centromeric histone H3 CENP-A homolog in C. tropicalis [85], was identified in a BLAST analysis using C. albicans CENP-A (CaCse4) as the query sequence against the Candida tropicalis genome [43]. The BLAST analysis revealed that the proteins with high scores (score >213) were the putative CENP-A homologue, CtCse4 (CTRG_02639.3), and histone H3 proteins (CTRG_04732.3, CTRG_00676.3 and CTRG_05645.3). The CtCse4 (Scnt 3: 1334129–1334845) is a 238-aa-long protein that shows 90% homology with the C-terminal histone fold domain of CaCse4 (S1A Fig). Similarly, CENP-C (Mif2), Nuf2, and Dad1 homologs of C. tropicalis were identified in a BLAST analysis. The CtMif2 (CTRG_05763.3) is a 523-aa-long protein (Scnt 9: 474053–475624+) with a conserved CENP-C box, which is identical in sequence between the CaMif2 and CtMif2 (S1A Fig). CtNUF2 (CTRG_05381.3) and CtDAD1 (CTRG_03625.3) encode 492-aa- and 99-aa-long proteins respectively. Both of these proteins show a high degree of sequence conservation in comparison to those of C. albicans (S1A Fig).