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For EGFR knockdown, two separate EGFR siRNA were purchased from Thermo Fisher Scientific (siRNA ID 43833) and Santa Cruz (sc-29301). Control siRNA was purchased from Thermo Fisher Scientific (AM4611). siRNAs were transfected into cells using oligofectamine (Invitrogen, 12252011). Cells were starved in the serum-free medium 24 hr after transfection and proceeded for transwell assay. LGR4 knockdown experiments were performed as previously described (13).
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