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CDC20 siRNA duplexes were transfected using Lipofectamine 3000 reagent (Invitrogen, USA). Cells were used for further analyses after transfection for 48 h. The sequences of the CDC20 siRNA duplexes are as follows: si1#: AACGGCAGGACUCCGGGCCGA, si2#: AAUGGCCAGUGGUGGUAAUGA.
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