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At the end of the treatment period, cells were washed twice with phosphate-buffered saline (PBS, 0.1 M, pH 7.4) and incubated for 8 min with 15 µg/mL FDA-AM in the dark, rinsed twice with PBS, and lysed with a Tris/HCl solution containing 1% sodium dodecyl sulfate. Fluorescence was measured with excitation at 485 nm and emission at 538 nm using a fluorescence microplate reader FL800TBI (Bio-Tek Instruments, Winooski, VT, USA). Experiments were carried out on 16 different wells from four independent experiments.
Copyright and License information: ©2017 Amri, Ghouili, Tonon, Amri and Masmoudi-Kouki.
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