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Isolation of rat adult ventricular cardiomyocytes

MM Marta Maiolino
PC Pasqualina Castaldo
VL Vincenzo Lariccia
SP Silvia Piccirillo
SA Salvatore Amoroso
SM Simona Magi
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One-month old male Wistar rats (Charles River, Lecco, Italy) were used for cardiomyocytes isolation. The animal protocol was approved by the Ethic Committee for Animal Experiments of the University Politecnica of Marche (Ref no. 721/2015-PR). All the experiments were conducted in strict accordance with the guidelines of the Italian Ministry of Health (D.L.116/92 and D.L.111/94-B). All efforts were made to minimize the number of animals used as well as their suffering.

Cardiomyocytes were isolated by Collagenase type II-CLS2 (Worthington Biochemical Corporation, Lakewood, NJ) digestion using a modified Langendorff perfusion system as previously described19,20 (See Supplementary Information for further details).

Copyright and License information: The Author(s) ©2017
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