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Peripheral blood, 4 ml, was obtained from each patient and control. Samples were centrifuged for 10 min at 3000 g to separate serum. Ethylene diamine tetraacetic acid (EDTA) anticoagulant tubes were used to collect another 2 ml venous blood samples from each participant. A blood DNA isolation kit was used to extract the peripheral white blood cell genome (MBI, USA). The subsequent DNA extraction procedures were performed according to the manufacturer instructions and DNA were stored in a refrigerator at -20 °C.
Copyright and License information: The Author(s). ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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