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Two milliliters of venous blood sample taken from patients was inoculated into 20 ml brain-heart infusion broth (BHI) (CM1135; Oxoid Ltd., England, UK). The culture bottles were incubated at 37 °C for 7 days and examined daily for evidence of bacterial growth, including turbidity and hemolysis. If bacterial growth was observed, a subculture was made after 24 h on blood agar (CM0271, Oxoid Ltd., England UK), Salmonella Shigella agar (SS) (CM0099, Oxoid Ltd., England, UK), and MacConkey agar (CM0007, Oxoid Ltd., England, UK).
Copyright and License information: The Author(s) ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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