Experimental Design

DP David Püschel
MJ Martina Janoušková
AV Alena Voříšková
HG Hana Gryndlerová
MV Miroslav Vosátka
JJ Jan Jansa
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In a greenhouse pot experiment, two model plant species, M. truncatula and M. sativa, were planted in two different substrates amended or not with mineral P-fertilizer to reach three levels of P availability for each of the substrates. All plants were inoculated with rhizobia compatible with the respective host plant species. Half of the plants were further inoculated with AM fungal isolate Rhizophagus irregularis ‘PH5,’ whereas the other plants grew without the AM fungus. The experiment was conducted in a fully factorial experimental design with five biological replicates per treatment, and thus it comprised 120 pots.

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