Cell lines, transfections and drug treatments

GD Grzegorz Dobrynin
TM Tom E. McAllister
KL Katarzyna B. Leszczynska
SR Shaliny Ramachandran
AK Adam J. Krieg
AK Akane Kawamura
EH Ester M. Hammond
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RKO, MCF7, MDA-MB-231, HCT116 and FaDuHRE-luc were cultured in Dulbecco’s modified Eagle’s medium (D-MEM) supplemented with 10% FBS and 1% penicillin/streptomycin. For siRNA experiments, cells were transfected with 10 nM siRNA using Lipofectamine RNAiMAX in OptiMEM minimal medium (Invitrogen). As a negative control, ON-TARGETplus Non-targetting Control Pool siRNA (Dharmacon, D-001810-10) was used. siKDM4A S1 (Ambion, ID: 148456), siKDM4A Q1-Q4 (Qiagen, 10274116), siKDM4B (Ambion, ID: 148507), siKDM4C (Ambion, ID: 108664), siKDM4D (Dharmacon, siGENOME SMARTpool D-020709-01 to 04). ML324 (Sigma-Aldrich, SML0741), JIB-04 (Sigma-Aldrich, SML0808) and IOX1 (Sigma Aldrich, SML0067).

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