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At the National Reference Laboratory for Rabies, direct FAT was routinely done using an anti-RABV nucleocapsid fluorescent conjugate (Bio-Rad, USA). For diagnostic purposes, PCR was also routinely performed using primers that target a portion of the RNA-dependent RNA polymerase-coding region. Extraction of viral RNA from the original fresh brain samples was done using QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturer’s instructions.
Copyright and License information: ©2016 Tricou et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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