Cell-Permeable C6 Cer Treatment

C₆ Cer (Biomol, USA), short for C₆ Cer, was dissolved in anhydrous DMSO to obtain 5 mg mL⁻¹ C₆ Cer stock solution. For Cer treatment, 10 sea cucumbers (100 ± 10 g) were injected with 100 μL of Cer at the concentration of 100 μg mL⁻¹. The sea cucumbers in the control group were given an injection of 100 μL diluted DMSO. Twenty-four hours after the injection, the treated and control coelomocytes were collected for further expression analysis.