Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Serum Level of Total Cholesterol (TC), Low Density Lipoprotein (LDL)/Very Low Density Lipoprotein (VLDL) Cholesterol, High Density Lipoprotein (HDL) Cholesterol, and Triglyceride
Ask a
question
Favorite
To measure serum TC, LDL/VLDL-C, HDL-C, and TG, commercial assay kits [HDL and LDL/VLDL Cholesterol Assay Kit, STA-391 (Cell Biolabs Inc., San Diego, CA, USA); Triglyceride Quantification Colorimetric/Fluorometric Kit, K622-100, Biovision Inc. (Milpitas, CA, USA)] were used according to manufacturer instructions. Absorbance at 570 nm was read with a PowerWave HT Microplate Spectrophotometer (BioTek Instruments Inc., Winooski, VT, USA). Sample cholesterol concentrations were determined by interpolation from a standard curve.
Copyright and License information: The Author(s). ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
