Male rats (n = 8 per group-Cohort 2) at 1500 h were anesthetized and injected with insulin (1U/kg; Humalog® #CAT: VL7510, Eli Lilly and Company, Indianapolis, USA) after 6-h fasting. A blood drop was collected from the tail to measure glucose levels (Accu-Chek; Roche Diagnostics) at 0, 15, 25, 35, 45, and 60 min after glucose injection. A blood sample was collected at 0 min to measure fasting glucose, insulin, leptin, and adiponectin.
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