Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Cell survival assay

AS Aimilia D. Sklirou
NG Nicolas Gaboriaud-Kolar
IP Issidora Papassideri
AS Alexios-Leandros Skaltsounis
IT Ioannis P. Trougakos
request Request a Protocol
ask Ask a question
Favorite

The effect of 6BIO on the viability of BJ cells was examined using the MTT assay. Briefly, cells were plated in flat-bottomed 96-well microplates and the next day they were incubated with different concentrations of 6BIO for 24 h. Afterwards, the medium was replaced by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich) dissolved at a final concentration of 1 mg/ml in serum-free, phenol red-free medium. The formed formazan crystals were then dissolved by isopropanol and the absorbance of the solution was measured at 570 nm wavelength. Survival of control cells was arbitrarily set to 100%.

Copyright and License information: The Author(s) ©2017
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A