Cell culture

YZ Yufan Zhu
ZY Zhiqiang Yang
YX Yuanlong Xie
MY Min Yang
YZ Yufeng Zhang
ZD Zhouming Deng
LC Lin Cai
request Request a Protocol
ask Ask a question
Favorite

Human osteosarcoma cell lines (143B and U2OS) and human osteoblast cell line hFOB 1.19 were obtained from the China Centre for Type Culture Collection (Wuhan, China). 143B cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% heat - inactivated FBS (Gibco) and 1% antibiotics in a 37 °C incubator with 5% CO2. U2OS cells were cultured in McCoy’s 5A medium (modified) when hFOB 1.19 was cultured in α-MEM (HyClone) and the other conditions were the same as those used for 143B cells.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A