Human osteosarcoma cell lines (143B and U2OS) and human osteoblast cell line hFOB 1.19 were obtained from the China Centre for Type Culture Collection (Wuhan, China). 143B cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% heat - inactivated FBS (Gibco) and 1% antibiotics in a 37 °C incubator with 5% CO2. U2OS cells were cultured in McCoy’s 5A medium (modified) when hFOB 1.19 was cultured in α-MEM (HyClone) and the other conditions were the same as those used for 143B cells.
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