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The use of clodronate-containing liposomes is a widely accepted approach to deplete monocytes/macrophages and microglia. Liposome-encapsulated clodronate liposomes were prepared as previously described (11). Intravenous applications of clodronate liposomes (0.5 ml per 100 g body weight) were administered via tail vein right before the ONT procedure (12) and 200 μg/mL of liposomal clodronate was used in the co-culture system.
Copyright and License information: ©2021 Geng, Lu, Guan, van Rooijen and Zhi
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