Cell counting for growth curves

HC Hsiao-Ying Chiu
YT Yeou-Guang Tsay
SH Shih-Chieh Hung
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All cells were seeded into 24-well culture plates (3 × 104/well), the cells were allowed to continue in culture for 24 hr, 48 hr, or 72 hr and then were counted under the microscope with trypan blue (93595, Sigma-Aldrich) exclusion. All assays were performed in triplicate.

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