Stereotaxic Virus Injection and Cannula Implantation.

EC Erin S. Calipari
RB Rosemary C. Bagot
IP Immanuel Purushothaman
TD Thomas J. Davidson
JY Jordan T. Yorgason
CP Catherine J. Peña
DW Deena M. Walker
SP Stephen T. Pirpinias
KG Kevin G. Guise
CR Charu Ramakrishnan
KD Karl Deisseroth
EN Eric J. Nestler
request Request a Protocol
ask Ask a question
Favorite

AAVdj-CaMKIIα-GCaMP6f-WPRE (for pan-neuronal recordings), AAVdj-EF1α-DIO-GCaMP6f-WPRE (for cell-type-specific recording), or AAV5/hSyn-DIO-hm4Di-mcherry [University of North Carolina at Chapel Hill (37), for DREADD inhibition studies] was infused into NAc core. Chronically implantable optic fibers (Doric Lenses) were positioned above the injection site.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A