Genome Sequencing, Assembly, Bioinformatics, and Statistics.

The K. rhaeticus iGEM genome was sequenced with an Illumina MiSeq using 250-bp paired-end reads, to a coverage of ∼400×. Reads were then downsampled to 100× coverage, assembled using the BugBuilder pipeline (50), quality-controlled, and annotated using Prokka with the full read set (51). All statistical tests were performed with Prism 6 (GraphPad Software). For details of genome sequencing and bioinformatics, see SI Appendix, Supplementary Methods.