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Carmine alum whole mount processing/staining
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Following fixation, whole mount mammary glands were washed, dehydrated through an alcohol series and defatted with toluene (Sigma-Aldrich, St. Louis MO). The glands were then stained overnight with carmine alum (Sigma-Aldrich), dehydrated through a series of alcohols and xylene, and then placed in k-pax heat-sealed bags (Fisher) with methyl salicylate (Fisher) to preserve them.
Copyright and License information: The Author(s). ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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