The mean phenotypic data from all the 170 individuals (two parents and 168 F2:3 progenies) were calculated for frequency distributions using function formula “NORMDIST” in Excel 2010.
Quantitative trait loci (QTL) were detected for each of the traits using the CIM (composite interval mapping) method implemented in Windows QTL Cartographer 2.5 (Wang S. et al., 2012). The LOD significance thresholds (P < 0.05) were analyzed by running 1,000 permutation tests. QTL regions were visualized by the program Matplotlib (version 1.4.3) in software Python version 2.7.10 (Sanner, 1999).
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