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Protein expression analysis by immunohistochemical staining
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Immunohistochemical (IHC) staining of tumor samples was carried out to assess the expression of phosphorylated EGF receptor (pEGFR), and EGFR downstream molecules, such as phosphorylated AKT (pAKT), P27 and m-TOR. PTEN expression was also analysed, which located in upstream of PI3K/AKT. Positive staining was defined as staining above background level in ≥10% of cancer cells.
Copyright and License information: The Author(s). ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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