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Compounds 2, 4 and 5 were evaluated for their in vitro activity against cancer cell lines by the National Cancer Institute (NCI) following its anticancer drug development programme based on automated sulforhodamine blue (SRB) cytotoxicity assay. The screening is a two-stage process, where after a first evaluation carried out against the full panel of cell lines at a single dose of 10 µM, the compounds exhibiting significant growth inhibition are tested at five concentration levels22.
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