Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Plant materials and observations

TK Takashi Kuromori
ES Eriko Sugimoto
HO Haruka Ohiraki
KY Kazuko Yamaguchi-Shinozaki
KS Kazuo Shinozaki
request Request a Protocol
ask Ask a question
Favorite

Plants were grown in soil under well-watered conditions at 22 ± 2 °C and 60–70% relative humidity under a 16/8-h light/dark cycle. The atabcg21 mutant was a T-DNA-tagged mutant obtained from the Arabidopsis Biological Resource Center (SAIL_786_G09). The atabcg22 mutant used in this study was reported previously as the atabcg22-2 (salk_113844) allele, and the srk2e and nced3 mutants have been described previously30.

Thermal images were captured using an infrared thermography device (T620; FLIR). Average temperatures were calculated by FLIR Tools. Water-loss experiments were performed using rosette leaves detached from 5-week-old plants, as described previously30.

Copyright and License information: The Author(s) ©2017
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A