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Mice were injected intraperitoneally with 4 μg α-galactosylceramide. Splenocytes were collected 4 h later and surface stained with CD1d-PBS57 and anti-B220 antibodies. iNKT cells were sorted as CD1d-PBS57+B220− population. RNA was extracted with RNeasy Protocol (Qiagen) and analyzed for gene expression profiling using Agilent Single color 26655 platform at MOgene (GSE73679).
Copyright and License information: ©2016
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